Vysis LSI CBFB Break Apart FISH Probe Kit

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The Vysis LSI CBFB Dual Color Break Apart Rearrangement fluorescence in situ hybridization (FISH) probe is intended to detect chromosomal rearrangements at the CBFB locus on chromosome 16q22.

The SpectrumRed probe is approximately 130 kb (chr16:65525674- 65655811; March 2006 assembly)5 and hybridizes centromeric to the inv(16) and t(16;16) breakpoint region. The SpectrumGreen probe is approximately 204 kb (chr16:65677619- 65881775; March 2006 assembly) and hybridizes telomeric to the breakpoint.

The expected pattern in a nucleus lacking inv(16) will be two fused red/green (yellow) signals (2F). The pattern in a nucleus containing an inv(16) results in separate red and green signals appearing on opposite arms of the inverted 16 chromosome. The pattern of t(16;16)(p13;q22) results in an adjacent or fused red/ green signal on the q arm of one of the 16 chromosomes and a green signal on the other arm of 16, while the 16 chromosome homolog will only contain the red signal on one arm.

Normal Hybridization: LSI CBFB Dual Color, Break Apart Rearrangement Probe hybridized to a cell exhibiting one red and one green signal. On the metaphase cell, contains the red signal on one arm and the green signal on the other arm.