Vysis CDKN2A / CEP 9 FISH Probe Kit

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This fluorescence in situ hybridization (FISH) probe is intended to detect deletion of the LSI CDKN2A (p16) probe target within the 9p21 chromosome region.

Alterations of the 9p21 locus including the tumor suppressor gene CDKN2A (p16) are implicated in different Meningiomas and Gliomas. Studies support the association of CDKN2A homozygous deletion with malignant progression and suggest that it is a marker of worse prognosis in anaplastic oligodendrogliomas. The Vysis LSI CDKN2A SpectrumOrange/CEP 9 SpectrumGreen Probes have been used in several cytogenetic studies to detect losses of the CDKN2A gene. Using this probe set as well as other relevant markers (eg, p53, RB1, 1p36, 19q13, all Vysis FISH probes), Kramar, et al investigated 82 samples from 81 patients with histologically confirmed glial tumors. In a study using the Vysis LSI CDKN2A SpectrumOrange/ CEP 9 SpectrumGreen Probes on 189 confirmed glioblastoma patients less than 50 years old, Korshunov, et al found 9p21 deletion to be correlated with an unfavorable prognosis.

Vysis LSI CDKN2A/CEP 9 Probes are provided in one vial as a mixture of the LSI CDKN2A (p16) probe labeled with SpectrumOrange and the CEP 9 probe labeled with SpectrumGreen. The LSI CDKN2A probe spans approximately 222 kb and contains a number of genes including MTAP, CDKN2A, and CDKN2B. The LSI CDKN2A contains a number of genetic loci including D9S1749, p16 (INK4B), p14 (ARF), D9S1748, p15(INK4B), and D9S1752. The CEP 9 SpectrumGreen probe hybridizes to alpha satellite sequences specific to chromosome 9


In a normal sample, the expected pattern for a nucleus hybridized with the Vysis LSI CDKN2A / CEP 9 Probe is the two orange, two green (2O2G) signal pattern. If a deletion at the 190 kb region covered by the LSI p16 probe occurs on one chromosome 9 homolog and both centromeres from chromosome 9 are retained, the one orange, two green (1O2G) signal pattern is expected.Very small deletions may occur that do not delete the entire LSI p16 probe target and therefore will not be detected.

Abnormal Hybridization: Vysis LSI CDKN2A / CEP 9 Probe hybridized to a nucleus exhibiting the one orange and two green signal (1O2G) pattern. One p16 gene locus is deleted and both chromosome 9 homologs are present as indicated by one orange and two green signals, respectively.