Molecular Catalog
Molecular Catalog

Vysis ETV6 Break Apart FISH Probe Kit

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Product Description
RESULTS OF HYBRIDIZATION
Product Description

The LSI ETV6 fluorescence in situ hybridization (FISH) probe kit is intended to detect chromosomal rearrangements involving the ETV6 gene located at chromosome 12p13.

The Vysis ETV6 Dual Color Break Apart Rearrangement Probe consists of a mixture of 2 FISH DNA probes. The approximately 486 kb SpectrumOrange-labeled probe lies telomeric to the ETV6 gene breakpoint region. The approximately 632 kb SpectrumGreen-labeled probe lies centromeric to the ETV6 gene breakpoint region..

RESULTS OF HYBRIDIZATION

Hybridization of this probe to interphase nuclei of normal cells is expected to produce two pair of overlapping, or nearly overlapping, orange and green (yellow fusion) signals.

The anticipated signal pattern in abnormal cells having a chromosomal breakpoint within the gap between the two probe targets on one chromosome 12 is one orange, one green, and one fusion signal. Other patterns may be observed if additional genetic alterations are present.

Normal Hybridization: Normal cell hybridization using the LSI ETV6 (TEL) (12p13) Dual Color, Break Apart Rearrangement Probe.
Abnormal Hybridization: Abnormal cell hybridization using the LSI ETV6 (TEL) (12p13) Dual Color, Break Apart Rearrangement Probe.
  • Vysis FISH
  • Abbott REAL-TIME PCR
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