Vysis LSI BCR/ABL Dual Color, Dual Fusion Translocation Probe Kit

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This fluorescence in situ hybridization (FISH) probe is intended to detect the t(9;22)(q34;q11.2) and complex or masked variants of t(9;22) that result in the BCR/ABL gene fusion. The probe may be used with metaphase chromosomes or interphase nuclei.

The approximately 671 kb SpectrumOrange ABL probe extends from a point centromeric of the arginosuccinate synthase gene (ASS1) to telomeric of the ABL1 gene on chromosome 9. The SpectrumGreen BCR probe spans a genomic distance of approximately 1.5 Mb. It begins within the variable segments of the IGL locus on chromosome 22 and ends at a point about 900 kb telomeric to the BCR gene. A 326 kb region immediately telomeric of the BCR gene is not present in the probe. Both BCR and ABL1 probes span the typical t(9;22) chromosomal breakpoints for their respective genes.

A nucleus lacking the t(9;22) translocation will exhibit the two orange, two green (2O2G) signal pattern. In a nucleus containing a simple balanced t(9;22), one orange and one green signal from the normal 9 and 22 chromosomes and two orange/green (yellow) fusion signals, one each from the derivative 9 and 22 chromosomes, will be observed (1O1G2F). In some instances, deletions may occur 3’ of the BCR breakpoint and/or 5’ of the ABL breakpoint resulting in either an ES (extra orange or green) signal pattern or a single fusion pattern.

Abnormal Hybridization: LSI BCR/ABL Dual Color, Dual Fusion Translocation Probe hybridized to a nucleus containing a simple balanced t(9;22). One orange, one green and two orange/green fusion signals are observed (1O1G2F).