Vysis LSI BCR/ABL Dual Color, Single Fusion Translocation Probe Kit

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The LSI BCR/ABL Dual Color, Single Fusion Translocation Probe is intended to identify the BCR/ABL gene fusion resulting from the t(9;22)(q34;q11.2) or other chromosomal rearrangements. The probe may be used with metaphase chromosomes or interphase nuclei.

The SpectrumOrange ABL1 probe extends from a point between ABL1 exons 4 and 5 and extends for approximately 190 kb toward the 9q telomere. This probe target flanks the expected chromosome 9 breakpoint in t(9;22)(q34;q11.2). The SpectrumGreen BCR probe begins between BCR exons 13 and 14 (major breakpoint cluster region [M-bcr] exons 3 and 4) and extends toward the chromosome 22 centromere for approximately 287 kb. The BCR probe flanks the expected M-bcr breakpoints, but spans the minor breakpoint cluster region (m-bcr) for t(9;22)(q34;q11.2).

A nucleus lacking the t(9;22) will exhibit the two orange, two green (2O2G) signal pattern. In a cell harboring the t(9;22), one orange, one green, and one orange/ green (yellow) fusion signal pattern (1O1G1F) will be observed. This simple probe design detects the 5’ BCR/3’ ABL gene fusion and is useful for detecting samples with a high percentage of cells possessing this translocation.

Normal Hybridization: LSI BCR/ABL Dual Color, Single Fusion Translocation Probe hybridized to a nucleus containing the t(9;22). One orange, one green and one fusion (IOIGIF) signal pattern is observed.