Vysis MDM2/CEP 12 FISH Probe Kit

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The Vysis MDM2/CEP 12 FISH Probe Kit is intended to detect the copy number of the LSI MDM2 probe target located at chromosome 12q15 using the fluorescence in situ hybridization (FISH) technique.

The approximately 209 kb (chr12:67,420,133-67,629,503; March 2006 assembly) SpectrumOrange probe spans MDM2 gene on 12q15. The SpectrumGreen CEP 12 probe hybridizes to alphoid sequences found within the centromere of chromosome 12 (12p11.1-q11).

Normal Hybridization: Nuclei or metaphase chromosome sets lacking the MDM2 amplification are expected to exhibit two orange and two green signals. Amplification of MDM2 would exhibit more than two orange signals and amplification of centromere 12 would exhibit more than two green signals.