Vysis LSI RPN1/MECOM DF FISH Probe Kit

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Product Description
Results of Hybridization
Product Description

The Vysis RPN1/MECOM DF FISH Probe Kit is intended to detect a fusion between the ribophorin I gene (RPN1) and the MDS1 and EVI1 complex locus gene (MECOM) using the fluorescence in situ hybridization (FISH) technique.

The approximately 580 kb (Chr3:129466532-130046908, March 2006 assembly, University of California, Santa Cruz [UCSC] Genome Browser) SpectrumGreen LSI RPN1 probe spans the RPN1 gene area on chromosome 3q21.3. The approximately 1.2 Mb (Chr3:169797344- 170950559, March 2006 assembly, UCSC Genome Browser) SpectrumOrange LSI MECOM probe encompasses the entire MECOM (MDS1 and EVI1 complex) locus on chromosome 3q26.2.

Results of Hybridization

The most frequently expected signal pattern of the Vysis LSI RPN1/MECOM Dual Color Dual Fusion Probes in abnormal specimens is 1 orange, 1 green, and 2 orange/green fusion signals. Other signal patterns may occur in abnormal specimens, and metaphase analysis may be helpful in characterization of such patterns.

The most commonly expected signal pattern of the Vysis LSI RPN1/ MECOM Dual Color Dual Fusion Probes in normal specimens is 2 orange and 2 green signals. Due to the proximity of the 2 probes on the q arm of chromosome 3, however, the orange and green signals may sometimes appear as a fusion in a normal nucleus. This effect can produce a pattern of 1 orange, 1 green, and 1 orange/green fusion signal or, more rarely, 2 orange/green fusion signals.

Abnormal Hybridization: Vysis LSI RPN1/ MECOM Dual Color Dual Fusion Probes hybridized to a nucleus containing a simple balanced t(3;3)(q21.3;q26.2). One orange, one green and two orange/green fusion signals are observed.
Normal Hybridization: Vysis LSI RPN1/ MECOM Dual Color Dual Fusion Probes hybridized to a nucleus containing non- rearranged RPN1 and MECOM regions. Two orange and two green signals are observed.

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