Vysis LSI TOP2A / CEP 17 FISH Probe Kit

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The Vysis LSI TOP2A/CEP 17 FISH Probe Kit is intended to determine TOP2A and chromosome 17 copy numbers, and hence can distinguish TOP2A amplification and deletion from chromosome 17 aneusomy.

The Vysis LSI TOP2A SpectrumOrange/CEP 17 SpectrumGreen Probes vial contains a mixture of 2 probes. The TOP2A probe is a single approximately 166 kb unique sequence probe direct labeled in SpectrumOrange, that hybridizes to the 17q21-q22 region of chromosome 17 and includes the 30 kb topoisomerase II-α gene. The CEP 17 probe hybridizes to alpha satellite DNA on chromosome 17 (17p11.1-q11.1) and is directly labeled in SpectrumGreen. This probe set is premixed in hybridization buffer.


In a cell with the normal quantity (two copies) of the TOP2A gene, two orange signals will be observed. If amplification or deletion of the TOP2A gene has occured, more or less than two signals will be present.