Vysis LSI D13S319 (13q14.3) SpectrumOrange Probe
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Vysis LSI D13S319 (13q14.3) SpectrumOrange DNA probe hybridizes to the band 13q14.3, locus D13S319 of human chromosome 13. The hybridized probe fluoresces with bright intensity both in interphase nuclei and on metaphase chromosomes. In interphase nuclei of normal cells, the probe generally appears as two distinct signals. Occasionally, it may appear as three or four signals depending upon the condensation of the DNA and the relative distances between chromatids. The signals may also appear diffuse or split. In a normal metaphase, LSI D13S319 may appear as one or two signals on each chromosome 13.
In a normal cell, the expected pattern for the LSI D13S319 probe is the two orange (2O) signal pattern. In a hybridized abnormal cell containing the deletion, a one orange (1O) signal pattern will be observed.
Normal Hybridization: LSI D13S319 Single Color Probe hybridized to a normal metaphase showing the two orange (2O) signal pattern.
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