Vysis LSI 13 (RB1) 13q14 SpectrumOrange Probe

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Vysis LSI 13 (RB1) 13q14 SpectrumOrange DNA probe hybridizes to band 13q14 of human chromosome 13. The hybridized probe fluoresces with bright intensity both in interphase nuclei and on metaphase chromosomes. In interphase nuclei of normal cells, the probe generally appears as two distinct signals. It may also appear as three or four signals depending upon the condensation of the DNA and the relative distances between chromatids. The signals may also appear diffuse or split. In a normal metaphase, the probe may appear as one or two signals on each chromosome 13.


In a normal cell, the expected result for a nucleus hybridized with the LSI 13 (RB1) probe is a two orange (2O) signal pattern. In a hybridized abnormal cell containing the deletion, a one orange (1O) signal pattern will be observed.

Normal Hybridization: LSI 13 (RB1) 13q14 Probe hybridized to a normal nucleus showing a two orange (2O) signal pattern