Vysis LSI 9q34 SpectrumAqua Probe

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Vysis LSI 9q34 SpectrumAqua DNA probe hybridizes to human chromosome 9q34. The hybridized probe fluoresces with moderate to bright intensity both in interphase nuclei and on metaphase chromosomes. In interphase nuclei of normal cells, the probe generally appears as two distinct signals. The probe may also appear as three or four signals, depending upon DNA condensation, and relative distance between chromatids. The signals may also appear as diffuse or split signals. In a normal metaphase, the probe may appear as one or two signals on each chromosome 9.


This probe is provided for those interested in assessing the deletion status of the 9q34 region of chromosome 9. In a normal cell with two intact copies of chromosome 9, two aqua signals will be observed. In an abnormal cell that has lost the 9q34 region of chromosome 9, fewer than two aqua signals will be observed.

Normal Hybridization: Result of the hybridization of the LSI 9q34 Probe as observed in a normal interphase cell.

Abnormal Hybridization: Abnormal cell hybridized with the LSI 9q34 Probe. The cell in this image shows deletion of one copy of the 9q34 region of chromosome 9 as indicated by the single aqua signal.