Vysis LSI BCL6 Dual Color Break Apart Rearrangement Probe

For more information, contact Abbott.



Vysis LSI BCL6 (ABR)* Dual Color Break Apart DNA probe hybridizes to the band 3q27. The 5’ BCL6 SpectrumOrange probe is ~349 kb in size and flanks the ABR of BCL6. The 3’ BCL6 SpectrumGreen probe is approximately 816kb in size and flanks the MBR region of BCL6. There is an approximate 265 kb gap between the two probes.

The hybridized probe fluoresces with moderate to bright intensity both in interphase nuclei and on metaphase chromosomes. In interphase nuclei of normal cells, the probe generally appears as two distinct signals (orange and green adjacent, or fused yellow). The two probes are separated by an approximately 265 kb gap that contains the entire BCL6 gene including the BCL6 breakpoint region, which may result in a gap between the orange and green signal in one or both normal, fused signals. Individual labs may wish to establish criteria for what constitutes truly split signals. Probe signals may also appear diffuse or split in interphase nuclei, depending upon the condensation of the DNA. In normal metaphase spreads, the probe may appear as one or two signals on each chromosome 3.

*ABR- Alternate Breakpoint Region


In a normal cell hybridized with the BCL6 probe, the expected signal pattern is two orange/green (2F) fusion signals.

Abnormal Hybridization: LSI BCL6 (ABR) Dual Color, Break Apart Rearrangement Probe hybridized to a nucleus with a translocation breakpoint involving the BCL6 gene showing a one orange, one green, one fusion (1O1G1F) pattern.