Vysis LSI BCR/ABL Dual Color Single Fusion Translocation Probes

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Vysis LSI BCR/ABL Dual Color Single Fusion Probes hybridize to chromosome 9q34 (abl oncogene -SpectrumOrange) and chromosome 22q11.2 (breakpoint cluster region - SpectrumGreen). The hybridized probe fluoresces with moderate to bright intensity both in interphase nuclei and on metaphase chromosomes.


A nucleus lacking the t(9;22) will exhibit the two orange, two green (2O2G) signal pattern. In a cell harboring the t(9;22), one orange, one green, and one orange/green (yellow) fusion signal pattern (1O1G1F) will be observed. This simple probe design detects the 5’ BCR/3’ ABL gene fusion and is useful for detecting samples with a high percentage of cells possessing this translocation.

Normal Hybridization: LSI BCR/ABL Dual Color, Single Fusion Translocation Probe hybridized to a nucleus containing the t(9;22). One orange, one green and one fusion (IOIGIF) signal pattern is observed.