Vysis LSI IGH/BCL2 Dual Color Dual Fusion Probes

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Vysis LSI IGH/BCL2 Dual Color Dual Fusion Probes hybridize to chromosome 14q32 (IGH SpectrumGreen) and chromosome 18q21 (BCL2 SpectrumOrange). The hybridized probe fluoresces with moderate to bright intensity both in interphase nuclei and on metaphase chromosomes.

The expected pattern in a normal nucleus hybridized with the LSI IGH/BCL2 probe is the two orange, two green signal pattern (2O2G). In a nucleus harboring a t(14;18), the most common pattern is one orange signal, one green signal (representing the normal homolog) and two orange/green (yellow) fusion signals representing the two derivative chromosomes resulting from the reciprocal translocation (1O1G2F pattern). Patterns other than 1O1G2F may be observed in some abnormal cells including instances of nuclei containing more than two fusion signals.

Abnormal Hybridization: LSI IGH/BCL2 Dual Color, Dual Fusion Translocation Probe hybridized to a nucleus containing the t(14;18) (q32;q21) showing the 1O1G2F signal pattern