Vysis LSI MLL Dual Color, Break Apart Rearrangement Probe

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Product Description
RESULTS OF HYBRIDIZATION
Product Description
Vysis LSI MLL Dual Color Break Apart Rearrangement Probe hybridizes to the band 11q23 (SpectrumGreen on the centromeric side and SpectrumOrange on the telomeric side of the MLL gene breakpoint). The hybridized probe fluoresces with moderate to bright intensity both in interphase nuclei and on metaphase chromosomes.

The LSI MLL Dual Color, Break Apart Rearrangement Probe consists of a 350 kb portion centromeric of the MLL gene breakpoint cluster region (bcr) labeled in SpectrumGreen and approximately 190 kb portion largely telomeric of the bcr labeled in SpectrumOrange. In approximately 25% of 11q23 translocations, a region beginning at the MLL breakpoint and extending distally is deleted. This probe can provide a better indication of the presence of the 11q23 translocation than a single color probe design.
RESULTS OF HYBRIDIZATION
The signal pattern observed in a cell lacking the MLL rearrangement is expected to show a two orange/green (yellow) fusion signal pattern (2F). In a cell possessing a MLL translocation, the expected pattern is one green/orange (yellow) fusion signal, one orange signal, and one green (1O1G1F) signal. With the MLL Dual Color, Break Apart Rearrangement Probe, a large deletion occurring distally from the MLL breakpoint might weaken or totally eliminate one of the two orange signals, potentially producing a FISH pattern characteristic of concomitant translocation and deletion, i.e., one orange/green fusion and one isolated green signal.
Abnormal Hybridization: LSI MLL Dual Color, Break Apart Rearrangement Probe hybridized to cells possessing a t(9:11) (p22;q23) and exhibiting the expected one orange, one green and one orange/green fusion signal pattern (1O1G1F).

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