Vysis LSI N-MYC (2p24.1) SpectrumOrange Probe

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Vysis LSI N-MYC (2p24.1) SpectrumOrange DNA probe hybridizes to the band region 2p23 - p24 of human chromosome 2. The hybridized probe fluoresces with bright intensity both in interphase nuclei and on metaphase chromosomes. In interphase nuclei of normal cells, the probe generally appears as two distinct signals. Occasionally, the probe may appear as three or four signals depending upon the condensation of the DNA and the relative distances between chromatids. The signals may also appear diffuse or split. In a normal metaphase, LSI N-myc may appear as one or two signals on each chromosome 2.

In a normal cell, the expected pattern for a nucleus hybridized with the LSI N-MYC Probe is the two orange (2O) signal pattern. In an abnormal cell containing amplification of the N-MYC oncogene, greater than two orange signals will be observed.

Abnormal Hybridization: LSI N-MYC Probe hybridized to a cell showing a high level of amplification of the N-MYC oncogene.