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The SpectrumOrange D15S10 probe hybridizes to 15q11-q13. The probe is ~150 kb in size and spans the D15S10 STS marker. The CEP 15 probe is labeled in SpectrumAqua and hybridizes to the centromeric region of chromosome 15 and acts as a control probe. The PML SpectrumGreen probe hybridizes to the 15q22-q24 region and is ~180 kb in size.
The probe may be used with metaphase chromosomes or interphase nuclei. The signals may also appear diffuse or split depending upon the condensation of the DNA and the relative distances between chromatids. The hybridized probe fluoresces with moderate to bright intensity both in interphase nuclei and on metaphase chromosomes.
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