Telomeric Regions: Rearrangements, Deletions, and Additions

Vysis ToTelVysion Multi-Color FISH Probe Kit

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Product Description

The Vysis ToTelVysion Multi-Color FISH Probe Kit is intended to detect chromosome-specific subtelomeric regions from metaphase regions of cultured peripheral blood using the fluorescence in situ hybridization (FISH) technique.

The Vysis ToTelVysion Multi-Color FISH Probe Kit consists of 41 TelVysion probes with CEP and LSI control probes to aid in interpretation (64 probes in total). The TelVysion probes are specific to: the p and q subtelomeres of the chromosomes 1 to 12 and 16 to 20; the q subtelomeres of the acrocentric chromosomes (13, 14, 15, 21, and 22); and the Xp/Yp and Xq/Yq pseudo-autosomal region subtelomeres. All but TelVysion 3q, TelVysion 4q, TelVysion 8p and TelVysion 17q cover a genomic region estimated to be within 300 kb of the first occurrence of substantial unique sequence for their respective chromosomes. TelVysion 3q, TelVysion 4q, TelVysion 8p and TelVysion 17q probes cover a genomic region estimated to be within 800 kb of the first occurrence of substantial unique sequence for their respective chromosomes.

In addition, several of the multi-color mixtures contain unique sequence LSI probes or alpha satellite CEP probes for purposes of identifying specific chromosomes within the mixtures. ToTelVysion probes are directly labeled with SpectrumOrange and/or SpectrumGreen fluorophores. The LSI and CEP probes are labeled with SpectrumAqua fluorophore. The probes in the ToTelVysion Probe Panel are provided in 15 mixtures, allowing analysis of 15 target areas on 3 slides prepared from 1 sample of cells in metaphase (5 hybridization target areas per slide, as selected by the user, based on cell distribution).

To learn more about Vysis ToTelVysion Multi-Color FISH Probe Kit please visit: https://www.molecular.abbott/int/en/products/genetics-genomics/vysis-totelvysion-probe-kit

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