Vysis LSI BCL2 Break Apart FISH Probe kit

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Product Description
RESULTS OF HYBRIDIZATION
Product Description
Vysis LSI BCL2 Dual Color Break Apart Rearrangement Probe hybridizes to the band 18q21 (SpectrumGreen on the 3’ (centromeric) side and SpectrumOrange on the 5’ (telomeric) side of the BCL 2 locus breakpoints). The hybridized probe fluoresces with moderate to bright intensity both in interphase nuclei and on metaphase chromosomes.
RESULTS OF HYBRIDIZATION
The anticipated signal pattern in abnormal cells having a chromosomal breakpoint within the gap between the two probe targets on one chromosome 18 is one orange, one green, and one fusion signal. Other patterns may be observed if additional genetic alterations are present.

Hybridization of this probe to interphase nuclei of normal cells is expected to produce two pair of overlapping, or nearly overlapping, orange and green (yellow fusion) signals.
Normal Hybridization: Normal cell hybridization using the LSI BCL2 Dual Color Break Apart Rearrangement Probe.
Abnormal Hybridization: Abnormal cell hybridization using the LSI BCL2 Dual Color Break Apart Rearrangement Probe.

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